IREB2 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [9D1-R] (HA610146)
Catalog# HA610146
Mouse Monoclonal to IREB2
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of IREB2 on different lysates with Mouse anti-IREB2 antibody (HA610146) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.