IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60] (HA723225)
Catalog# HA723225
Rabbit Monoclonal to IRE1
Tested for
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WB
Reacts with
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Human
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Mouse
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Rat
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of IRE1 on different lysates with Rabbit anti-IRE1 antibody (HA723225) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: C6 cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723225) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: C6 cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723225) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.