PRODUCT CODE: ET1611-11

IRAKM Recombinant Rabbit Monoclonal Antibody [SN64-07] (ET1611-11)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

Western blot analysis of IRAKM on different lysates using anti-IRAKM antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Human placenta       <br />
Lane 2: Human thyroid
  • Western blot analysis of IRAKM on different lysates using anti-IRAKM antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Human placenta       <br />
Lane 2: Human thyroid
Western blot analysis of IRAKM on different lysates using anti-IRAKM antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human placenta
Lane 2: Human thyroid

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

IRAKM Recombinant Rabbit Monoclonal Antibody [SN64-07] (ET1611-11)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue, human thyroid tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN64-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

61 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

IRAKM

SYNONYMS

ASRT5 antibody; IL 1 receptor associated kinase M antibody; IL-1 receptor-associated kinase M antibody; IL1 receptor associated kinase M antibody; Interleukin 1 receptor associated kinase 3 antibody; Interleukin 1 receptor associated kinase M antibody; Interleukin-1 receptor-associated kinase 3 antibody; IRAK M antibody; IRAK-3 antibody; IRAK-M antibody; Irak3 antibody; IRAK3_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

TISSUE SPECIFICITY

Expressed predominantly in peripheral blood lymphocytes.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway. An IL-1-dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFkB activation. A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkB. IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFkB activation. IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells. IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1.