IRAK4 Recombinant Rabbit Monoclonal Antibody [JE41-77] (HA721347)
Catalog# HA721347
Rabbit Monoclonal to IRAK4
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (HA721347) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: THP-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 22 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721347) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: THP-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 22 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721347) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.