IRAK2 Recombinant Rabbit Monoclonal Antibody [JG36-23] (ET7108-29)
Catalog# ET7108-29
Rabbit Monoclonal to IRAK2
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: K-562 cell lysate
Lane 2: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-29) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
IRAK2 Recombinant Rabbit Monoclonal Antibody [JG36-23] (ET7108-29)
Immunogen
Recombinant protein within human irak2 aa 30-270 / 625.
Host
Rabbit
Positive Control
K-562 cell lysate, PC-12 cell lysate, K-562, RAW264.7, C6, human prostate tissue, human spleen tissue, mouse prostate tissue, mouse spleen tissue, rat prostate tissue, rat spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG36-23
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
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IP
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1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 69 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
IRAK2
SYNONYMS
il1rak2 antibody;Interleukin 1 receptor associated kinase 2 antibody;Interleukin 1 receptor associated kinase like 2 antibody;Interleukin-1 receptor-associated kinase-like 2 antibody;IRAK 2 antibody;IRAK-2 antibody;Irak2 antibody;IRAK2_HUMAN antibody;MGC150550 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
TISSUE SPECIFICITY
Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. IRAK2 has been shown to interact with TRAF6 and Myd88.
CITATIONS
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Yunfeng Shi,et al
IRAK2 overexpression restrains prostate cancer progression by regulation of TRAF6 ubiquitination
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IHC
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Human
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Citation
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