PRODUCT CODE: ET7108-29

IRAK2 Recombinant Rabbit Monoclonal Antibody [JG36-23] (ET7108-29)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution.<br />
 Positive control:<br />
 Line 1: PC-3M <br />
        Line 2: A549<br />
 Line 3: HepG2
  • Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution.<br />
 Positive control:<br />
 Line 1: PC-3M <br />
        Line 2: A549<br />
 Line 3: HepG2
  • ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution.
Positive control:
Line 1: PC-3M
Line 2: A549
Line 3: HepG2

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

IRAK2 Recombinant Rabbit Monoclonal Antibody [JG36-23] (ET7108-29)

Immunogen

Recombinant protein within n-terminal human irak2 .

Host

Rabbit

Positive Control

PC-3M, A549, HepG2, Jurkat, human tonsil tissue, human prostate cancer tissue, human heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG36-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

69 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

IRAK2

SYNONYMS

il1rak2 antibody; Interleukin 1 receptor associated kinase 2 antibody; Interleukin 1 receptor associated kinase like 2 antibody; Interleukin-1 receptor-associated kinase-like 2 antibody; IRAK 2 antibody; IRAK-2 antibody; Irak2 antibody; IRAK2_HUMAN antibody; MGC150550 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

TISSUE SPECIFICITY

Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. IRAK2 has been shown to interact with TRAF6 and Myd88.