PRODUCT CODE: ET1611-57

Integrin alpha 2 Recombinant Rabbit Monoclonal Antibody [SN0752] (ET1611-57)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: A431 cell lysate
  • Western blot analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: A431 cell lysate
  • All lanes: Western blot analysis of Integrin alpha 2 with anti-Integrin alpha 2 antibody [SN0752] (ET1611-57) at 1:1,000 dilution.<br />
Lane 1/2: Wild-type Hela whole cell lysate (20 µg).<br />
Lane 3/4: Integrin alpha 2 fragment 1 knockdown Hela whole cell lysate (20 µg).<br />
Lane 5/6: Integrin alpha 2 fragment 2 knockdown Hela whole cell lysate (20 µg).<br />
<br />
ET1611-57 was shown to specifically react with Integrin alpha 2 in wild-type Hela cells. Weakened bands were observed when Integrin alpha 2 knockdown samples were tested. Wild-type and Integrin alpha 2 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1611-57, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Integrin alpha 2 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-57, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Integrin alpha 2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-57, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Integrin alpha 2 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-57, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: A431 cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Integrin alpha 2 Recombinant Rabbit Monoclonal Antibody [SN0752] (ET1611-57)

Immunogen

Synthetic peptide within human integrin alpha 2 aa 1,132-1,181 / 1,181.

Host

Rabbit

Positive Control

MCF-7 cell lysate, A431 cell lysate, A431, MCF-7, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, mouse colon tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN0752

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

150 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Integrin alpha 2

SYNONYMS

BR antibody; CD 49b antibody; CD49 antigen like family member B antibody; CD49 antigen-like family member B antibody; CD49b antibody; CD49b antigen antibody; Collagen receptor antibody; DX5 antibody; Glycoprotein Ia deficiency included antibody; GP Ia antibody; GP Ia deficiency, included antibody; GPIa antibody; HPA 5 included antibody; HPA5 included antibody; Human platelet alloantigen system 5 antibody; Integrin alpha 2 antibody; Integrin alpha-2 antibody; Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody; ITA2_HUMAN antibody; ITGA2 antibody; Platelet alloantigen Br(a), included antibody; Platelet antigen Br antibody; Platelet glycoprotein GPIa antibody; Platelet glycoprotein Ia antibody; Platelet glycoprotein Ia/IIa antibody; Platelet membrane glycoprotein Ia antibody; Platelet receptor for collagen, deficiency of, included antibody; Very late activation protein 2 receptor alpha 2 subunit antibody; VLA 2 alpha chain antibody; VLA 2 antibody; VLA 2 subunit alpha antibody; VLA-2 subunit alpha antibody; VLA2 antibody; VLA2 receptor alpha 2 subunit antibody; VLAA2 antibody

SEQUENCE SIMILARITIES

Belongs to the integrin alpha chain family.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The sixteen α and eight β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counter-receptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. Integrin α2 is responsible for adhesion of platelets and other cells to collagens. Modulation of collagen and collagenase gene expression force generation and organization of newly synthesized extracellular matrix.

CITATIONS

  • Professor Weiguo Lu

    FAM83A exerts tumor-suppressive roles in cervical cancer by regulating integrins