PRODUCT CODE: ET1701-97

Insulin degrading enzyme Recombinant Rabbit Monoclonal Antibody [JJ0949] (ET1701-97)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of Insulin degrading enzyme on different lysates using anti-Insulin degrading enzyme antibody at 1/1,000 dilution.<br />
Positive control: <br />
  Lane 1: zebrafish <br />
  Lane 2: Hela <br />
  Lane 3: K562
  • Western blot analysis of Insulin degrading enzyme on different lysates using anti-Insulin degrading enzyme antibody at 1/1,000 dilution.<br />
Positive control: <br />
  Lane 1: zebrafish <br />
  Lane 2: Hela <br />
  Lane 3: K562
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.
  • Western blot analysis of Insulin degrading enzyme on hybrid  fish (crucian-carp) heart tissue lysate using anti-Insulin degrading enzyme antibody at 1/500 dilution.
Western blot analysis of Insulin degrading enzyme on different lysates using anti-Insulin degrading enzyme antibody at 1/1,000 dilution.
Positive control:
Lane 1: zebrafish
Lane 2: Hela
Lane 3: K562

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Insulin degrading enzyme Recombinant Rabbit Monoclonal Antibody [JJ0949] (ET1701-97)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Hela, zebrafish, human liver cancer tissue, human colon cancer tissue, human gastric carcinoma tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0949

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

118 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Insulin degrading enzyme

SYNONYMS

Abeta-degrading protease antibody; FLJ35968 antibody; Ide antibody; IDE_HUMAN antibody; Insulin protease antibody; Insulin-degrading enzyme antibody; Insulinase antibody; Insulysin antibody; OTTHUMP00000020097 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M16 family.

TISSUE SPECIFICITY

Detected in brain and in cerebrospinal fluid (at protein level).

POST-TRANSLATIONAL MODIFICATION

The N-terminus is blocked.

SUBCELLULAR LOCATION

Cytoplasm, Cell membrane, Secreted.

FUNCTION

Insulin degrading enzyme (IDE), also known as insulinase, insulin protease or insulysin, cleaves the peptide hormone insulin with consequences for insulin response and resistance. IDE is a highly conserved thiol metalloprotease with ubiquitous expression including the brain. Since its discovery, IDE has been shown to degrade a variety of bioactive peptides. Recently the finding that IDE clears intracellular and extracellular amyloid products has spurred research into the link between IDE function and human neurodegenerative disease such as Alzheimer’s.