The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications
-
IHC-P
-
FC
-
ELISA
REACTIVITY
-
Human
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Insulin B Chain Rabbit Polyclonal Antibody (0807-11)
Immunogen
Full length human insulin b chain protein.
Host
Rabbit
Positive Control
Rat pancreas tissue, human pancreas tissue, HepG2, mouse pancreas tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
PURIFICATION
Immunogen affinity purified.
MOLECULAR WEIGHT
12 kDa
Isotype
IgG
APPLICATION DILUTION
-
IHC-P
-
1:200-1:10,000
-
FC
-
1:50-1:100
-
ELISA
-
1:5000
TARGET
UNIPROT #
PROTEIN NAME
Insulin B Chain
SYNONYMS
IDDM antibody;IDDM1 antibody;IDDM2 antibody;ILPR antibody;ins antibody;INS_HUMAN antibody;Insulin A chain antibody;Insulin B chain antibody;IRDN antibody;MODY11 antibody;Preproinsulin antibody;Proinsulin antibody;Proinsulin precursor antibody
SEQUENCE SIMILARITIES
Belongs to the insulin family.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Insulin is a hormone with extensive effects on both metabolism and several other body systems. It causes most of the body's cells to take up glucose from the blood (including liver, muscle, and fat tissue cells), storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.