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PRODUCT CODE: 0807-11

Insulin B Chain Rabbit Polyclonal Antibody (0807-11)

Applications

  • IHC-P

  • FC

  • ELISA

REACTIVITY

  • Human

  • Rat

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Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Insulin B Chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (0807-11, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • IHC-P

  • FC

  • ELISA

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Insulin B Chain Rabbit Polyclonal Antibody (0807-11)

Immunogen

Full length human insulin b chain protein.

Host

Rabbit

Positive Control

Mouse pancreas tissue, human pancreas tissue, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:200-1:2,000

  • FC

  • 1:50-1:100

  • ELISA

  • 1:5000

TARGET

UNIPROT #

P01308

PROTEIN NAME

Insulin B Chain

SYNONYMS

IDDM antibody; IDDM1 antibody; IDDM2 antibody; ILPR antibody; ins antibody; INS_HUMAN antibody; Insulin A chain antibody; Insulin B chain antibody; IRDN antibody; MODY11 antibody; Preproinsulin antibody; Proinsulin antibody; Proinsulin precursor antibody

SEQUENCE SIMILARITIES

Belongs to the insulin family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Insulin is a hormone with extensive effects on both metabolism and several other body systems. It causes most of the body's cells to take up glucose from the blood (including liver, muscle, and fat tissue cells), storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.