Insulin Mouse Monoclonal Antibody [A6-6] (EM80714)
Catalog# EM80714
Mouse Monoclonal to Insulin
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IHC-P
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ELISA
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IF-Tissue
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IHC-Fr
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Human
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Mouse
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Rat
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Glucagon (ET1702-20, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Insulin Mouse Monoclonal Antibody [A6-6] (EM80714)
Immunogen
Synthetic peptide within human insulin aa 25-54.
Host
Mouse
Positive Control
Human pancreas tissue, mouse pancreas tissue, rat pancreas tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A6-6
RRID
APPLICATION DILUTION
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IHC-P
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1:2,000
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ELISA
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1:10,000
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IF-Tissue
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1:200
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IHC-Fr
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 12 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Insulin
SYNONYMS
IDDM antibody;IDDM1 antibody;IDDM2 antibody;ILPR antibody;ins antibody;INS_HUMAN antibody;Insulin A chain antibody;Insulin B chain antibody;IRDN antibody;MODY10 antibody;Preproinsulin antibody;Proinsulin antibody;Proinsulin precursor antibody
SEQUENCE SIMILARITIES
Belongs to the insulin family.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Insulin is a hormone with extensive effects on both metabolism and several other body systems. It causes most of the body's cells to take up glucose from the blood (including liver, muscle, and fat tissue cells), storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.
CITATIONS
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Zhou Yihan,et al
Reg2 treatment is protective but the induced Reg2 autoantibody is destructive to the islets in NOD mice
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IHC
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IF
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Mouse
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Citation
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Xu, H., Du, X., Xu, J., Zhang, Y., Tian, Y., Liu, G., Wang, X., Ma, M., Du, W., Liu, Y., Dai, L., Huang, W., Tong, N., Wei, Y., & Fu, X.
Pancreatic β cell microRNA-26a alleviates type 2 diabetes by improving peripheral insulin sensitivity and preserving β cell function. PLoS biology, 18(2), e3000603.
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IHC-P
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Mouse
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Citation
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Regional differences in islet amyloid deposition in the residual pancreas with new-onset diabetes secondary to pancreatic ductal adenocarcinoma
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IHC
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Human
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Citation
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Zhang, X., Deng, D., Cui, D., Liu, Y., He, S., Zhang, H., Xie, Y., Yu, X., Yang, S., Chen, Y., & Su, Z.
Cholesterol Sulfate Exerts Protective Effect on Pancreatic β-Cells by Regulating β-Cell Mass and Insulin Secretion
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IF
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Mouse
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Citation
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The protective role of shenqi compound in type 2 diabetes: A comprehensive investigation of pancreatic β-cell function and mass
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WB
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Rat
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Citation
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He, S., Yu, X., Cui, D., Liu, Y., Yang, S., Zhang, H., Hu, W., & Su, Z.
Nuclear factor-Y mediates pancreatic β-cell compensation by repressing reactive oxygen species-induced apoptosis under metabolic stress
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IHC
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Mouse
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Citation
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