PRODUCT CODE: EM1701-33

Insulin Mouse Monoclonal Antibody [5E1] (EM1701-33)

  • IVD–IHC

Applications

  • WB

  • ELISA

  • IHC-P

REACTIVITY

  • Human

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin antibody. Counter stained with hematoxylin.

Applications

  • WB

  • ELISA

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Insulin Mouse Monoclonal Antibody [5E1] (EM1701-33)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

5E1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG1

APPLICATION DILUTION

  • IHC-P

  • 1:2,000-1:10,000

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

Insulin

SYNONYMS

IDDM antibody; IDDM1 antibody; IDDM2 antibody; ILPR antibody; ins antibody; INS_HUMAN antibody; Insulin A chain antibody; Insulin B chain antibody; IRDN antibody; MODY10 antibody; Preproinsulin antibody; Proinsulin antibody; Proinsulin precursor antibody

SEQUENCE SIMILARITIES

Belongs to the insulin family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Insulin is a secreted peptide hormone that elicits metabolic effects such as increases in glucose uptake and glycogen synthesis leading to a decrease in blood glucose concentration. Insulin is first formed as a precursor molecule, preproinsulin, which is later cleaved to proinsulin and finally to the mature insulin hormone. Mature insulin consists of 51 amino acids, contained within an A chain and a B chain that are connected by two disulfide bridges. It increases cell permeability to monosaccharides, amino acids and fatty acids. Insulin is secreted by the pancreas at basal levels in the absence of exogenous stimuli, with secretion increasing in response to glucose. Insulin action is effected by the binding of insulin to cell-surface receptors on the target cell membrane. Defects of insulin are the cause of hyperproinsulinemia and of type-II diabetes mellitus.