PRODUCT CODE: ET7108-49

ILF3 Recombinant Rabbit Monoclonal Antibody [JG37-63] (ET7108-49)

  • Recombinant

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution.
  • Western blot analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution.
  • ICC staining ILF3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ILF3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ILF3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with ILF3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ILF3 Recombinant Rabbit Monoclonal Antibody [JG37-63] (ET7108-49)

Immunogen

Recombinant protein of n-terminal human ilf3.

Host

Rabbit

Positive Control

Rat heart tissue lysate, 293, A431, SH-SY-5Y, SiHa, human tonsil tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG37-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75/95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

ILF3

SYNONYMS

CBTF antibody; Double stranded RNA binding protein 76 antibody; Double-stranded RNA-binding protein 76 antibody; DRBF antibody; DRBP76 antibody; ILF3 antibody; ILF3_HUMAN antibody; Interleukin enhancer binding factor 3 antibody; Interleukin enhancer-binding factor 3 antibody; M phase phosphoprotein 4 antibody; M-phase phosphoprotein 4 antibody; MPHOSPH4 antibody; MPP4 antibody; NF AT 90 antibody; NF-AT-90 antibody; NF110 antibody; NF90 antibody; NFAR antibody; Nuclear factor associated with dsRNA antibody; Nuclear factor of activated T cells 90 kDa antibody; Nuclear factor of activated T-cells 90 kDa antibody; TCP80 antibody; Translational control protein 80 antibody

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Thr-188 and Thr-315 by PKR in response to certain RNA viruses. This phosphorylation results in the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3 where it can bind to viral RNAs and impede viral replication.; Methylated by protein arginine N-methyltransferase 1.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

ILF3 (interleukin enhancer binding factor 3) or TCP80 (translational control protein 80), is a ubiquitously expressed nuclear protein that exists in a heterodimer with NF45. NF90 contains two DRBM (double-stranded RNA(dsRNA-)-binding) domains and one DZF domain and, in association with NF45 primarily participates in the regulation of IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES). NF45 and NF90 belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. Due to alternative splicing events, six isoforms exist for NF90, namely NFAR-2 (or ILF3-E), NFAR-1 (or DRBP76), isoform 3, DRBP76α (or ILF3-A), DRBP76δ (also known as DRBP76γ or ILF3-C) and isoform 6.