PRODUCT CODE: ER1901-61

IL8 Rabbit Polyclonal Antibody (ER1901-61)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of IL8 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-61, 1/200) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of IL8 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-61, 1/200) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-61, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-IL8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-61, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of IL8 was done on AGS cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-61, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of IL8 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-61, 1/200) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL8 Rabbit Polyclonal Antibody (ER1901-61)

Immunogen

Recombinant protein within human il8 aa 1-99 / 99.

Host

Rabbit

Positive Control

Mouse stomach tissue lysates, human tonsil tissue, human skeletal muscle tissue, AGS.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • IHC-P

  • 1:50-1:100

  • ICC

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

IL8

SYNONYMS

(Ala-IL-8)77 antibody; (Ser-IL-8)72 antibody; 9E3 antibody; Beta thromboglobulin like protein antibody; C-X-C motif chemokine 8 antibody; CEF-4 antibody; chemokine, CXC motif, ligand 8 antibody; CXCL8 antibody; Emoctakin antibody; GCP-1 antibody; GCP/IL-8 protein I antibody; GCP/IL-8 protein II antibody; GCP/IL-8 protein III antibody; GCP/IL-8 protein IV antibody; GCP/IL-8 protein V antibody; GCP/IL-8 protein VI antibody; GCP1 antibody; Granulocyte chemotactic protein 1 antibody; IL-8 antibody; IL-8(1-77) antibody; IL-8(9-77) antibody; IL8 antibody; IL8/NAP1 form I antibody; IL8/NAP1 form II antibody; IL8/NAP1 form III antibody; IL8/NAP1 form IV antibody; IL8/NAP1 form V antibody; IL8/NAP1 form VI antibody; IL8_HUMAN antibody; Inteleukin 8 antibody; LECT antibody; LUCT antibody; Lymphocyte-derived neutrophil-activating factor antibody; LYNAP antibody; MDNCF antibody; MDNCF-b antibody; MDNCF-c antibody; MONAP antibody; Monocyte derived neutrophil activating peptide antibody; Monocyte derived neutrophil chemotactic factor antibody; Monocyte-derived neutrophil chemotactic factor antibody; Monocyte-derived neutrophil-activating peptide antibody; NAF antibody; NAP 1 antibody; NAP-1 antibody; NAP1 antibody; Neutrophil activating peptide 1 antibody; Neutrophil activating protein 1 antibody; Neutrophil-activating factor antibody; Neutrophil-activating protein 1 antibody; Protein 3 10C antibody; Protein 3-10C antibody; SCYB 8 antibody; SCYB8 antibody; Small inducible cytokine subfamily B member 8 antibody; T cell chemotactic factor antibody; T-cell chemotactic factor antibody; TSG 1 antibody; TSG1 antibody

SEQUENCE SIMILARITIES

Belongs to the intercrine alpha (chemokine CxC) family.

POST-TRANSLATIONAL MODIFICATION

Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.; Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

IL-8, also known as neutrophil chemotactic factor, has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also stimulates phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis (e.g. histamine release), and the respiratory burst.IL-8 can be secreted by any cells with toll-like receptors that are involved in the innate immune response. Usually, it is the macrophages that see an antigen first, and thus are the first cells to release IL-8 to recruit other cells. Both monomer and homodimer forms of IL-8 have been reported to be potent inducers of the chemokine receptors CXCR1 and CXCR2. The homodimer is more potent, but methylation of Leu25 can block the activity of homodimers.