IL-6 Mouse Monoclonal Antibody [11-D4] (EM1701-58)
Catalog# EM1701-58
Mouse Monoclonal to IL-6
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WB
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IHC-P
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IF-Cell
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ELISA
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Human
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Mouse
Specifications
Product Type
Mouse monoclonal primary
Product Name
IL-6 Mouse Monoclonal Antibody [11-D4] (EM1701-58)
Immunogen
Recombinant protein within mouse il-6 aa 1-211 / 211.
Host
Mouse
Positive Control
Recombinant protein lysates, Hela, NIH/3T3, PC-3M, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
11-D4
RRID
APPLICATION DILUTION
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WB
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1:500
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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ELISA
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1:5,000-1:10,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
24 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
IL-6
SYNONYMS
Interleukin BSF 2 antibody;B cell differentiation factor antibody;B cell stimulatory factor 2 antibody;B-cell stimulatory factor 2 antibody;BSF 2 antibody;BSF-2 antibody;BSF2 antibody;CDF antibody;CTL differentiation factor antibody;Hepatocyte stimulatory factor antibody;HGF antibody;HSF antibody;Hybridoma growth factor antibody;Hybridoma growth factor Interferon beta-2 antibody;IFN-beta-2 antibody;IFNB2 antibody;IL 6 antibody;IL-6 antibody;IL6 antibody;IL6_HUMAN antibody;Interferon beta 2 antibody;Interferon beta-2 antibody;Interleukin 6 antibody;Interleukin 6 (interferon beta 2) antibody;Interleukin BSF 2 antibody;Interleukin-6 antibody
SEQUENCE SIMILARITIES
Belongs to the IL-6 superfamily.
POST-TRANSLATIONAL MODIFICATION
N- and O-glycosylated.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1β and TNFα act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6-specific α chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.
CITATIONS
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Shao, F., Liu, R., Tan, X., Zhang, Q., Ye, L., Yan, B., Zhuang, Y., & Xu, J.
MSC Transplantation Attenuates Inflammation, Prevents Endothelial Damage and Enhances the Angiogenic Potency of Endogenous MSCs in a Model of Pulmonary Arterial Hypertension
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WB
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Hen
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Citation
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