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PRODUCT CODE: EM1701-58

IL6 Mouse Monoclonal Antibody [11-D4] (EM1701-58)

Applications

  • WB

  • IHC-P

  • ICC

  • ELISA

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of IL6 on recombinant protein using anti-IL6 antibody at 1/1,000 dilution.
  • Western blot analysis of IL6 on recombinant protein using anti-IL6 antibody at 1/1,000 dilution.
  • ICC staining IL6 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IL6 (green) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IL6 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL6 antibody. Counter stained with hematoxylin.
Western blot analysis of IL6 on recombinant protein using anti-IL6 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • ELISA

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

IL6 Mouse Monoclonal Antibody [11-D4] (EM1701-58)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Recombinant protein lysate, Hela, NIH-3T3, PC-3M, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

11-D4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • ELISA

  • 1:5,000-1:10,000

TARGET

UNIPROT #

P05231

PROTEIN NAME

IL6

SYNONYMS

Interleukin BSF 2 antibody; B cell differentiation factor antibody; B cell stimulatory factor 2 antibody; B-cell stimulatory factor 2 antibody; BSF 2 antibody; BSF-2 antibody; BSF2 antibody; CDF antibody; CTL differentiation factor antibody; Hepatocyte stimulatory factor antibody; HGF antibody; HSF antibody; Hybridoma growth factor antibody; Hybridoma growth factor Interferon beta-2 antibody; IFN-beta-2 antibody; IFNB2 antibody; IL 6 antibody; IL-6 antibody; IL6 antibody; IL6_HUMAN antibody; Interferon beta 2 antibody; Interferon beta-2 antibody; Interleukin 6 antibody; Interleukin 6 (interferon beta 2) antibody; Interleukin BSF 2 antibody; Interleukin-6 antibody

SEQUENCE SIMILARITIES

Belongs to the IL-6 superfamily.

POST-TRANSLATIONAL MODIFICATION

N- and O-glycosylated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1β and TNFα act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6-specific α chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.