PRODUCT CODE: ET7106-61

IL13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89] (ET7106-61)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of IL13 Receptor alpha 1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of IL13 Receptor alpha 1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • ICC staining of IL13 Receptor alpha 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-61, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL13 Receptor alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of IL13 Receptor alpha 1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

IL13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89] (ET7106-61)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, HepG2, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU38-89

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50/40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

IL13 Receptor alpha 1

SYNONYMS

bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody; Cancer/testis antigen 19 antibody; CD213a1 antibody; CD213A1 Antigen antibody; CT 19 antibody; CT19 antibody; I13R1_HUMAN antibody; IL 13 receptor subunit alpha 1 antibody; IL 13R alpha 1 antibody; IL 13R subunit alpha 1 antibody; IL 13Ra antibody; IL 13RA1 antibody; IL-13 receptor subunit alpha-1 antibody; IL-13R subunit alpha-1 antibody; IL-13R-alpha-1 antibody; IL-13RA1 antibody; IL13 receptor alpha 1 chain antibody; IL13R antibody; IL13RA antibody; Il13ra1 antibody; Interleukin 13 receptor alpha 1 antibody; Interleukin 13 receptor alpha 1 chain antibody; interleukin 13 receptor subunit alpha 1 antibody; Interleukin-13 receptor subunit alpha-1 antibody; NR 4 antibody; NR4 antibody

SEQUENCE SIMILARITIES

Belongs to the type I cytokine receptor family. Type 5 subfamily.

TISSUE SPECIFICITY

Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Interleukin 13 receptor, alpha 1, also known as IL13RA1 and CD213A1 (cluster of differentiation 213A1), is a human gene. Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.