PRODUCT CODE: ER1803-31

IL12B Rabbit Polyclonal Antibody (ER1803-31)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis of IL12B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: Hela cell lysates<br />
Lane 2: Mouse placenta tissue lysates
  • Western blot analysis of IL12B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: Hela cell lysates<br />
Lane 2: Mouse placenta tissue lysates
Western blot analysis of IL12B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysates
Lane 2: Mouse placenta tissue lysates

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL12B Rabbit Polyclonal Antibody (ER1803-31)

Immunogen

Synthetic peptide within mouse il12b aa 220-300.

Host

Rabbit

Positive Control

Hela, Mouse placenta tissue lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

IL12B

SYNONYMS

CLMF antibody; CLMF p40 antibody; CLMF2 antibody; Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody; IL 12B antibody; IL-12 subunit p40 antibody; IL-12B antibody; IL12 p40 antibody; IL12B antibody; IL12B_HUMAN antibody; IMD28 antibody; IMD29 antibody; Interleukin 12 beta chain antibody; Interleukin 12 p40 antibody; Interleukin-12 subunit beta antibody; NK cell stimulatory factor chain 2 antibody; NKSF antibody; NKSF2 antibody

SEQUENCE SIMILARITIES

Belongs to the IL-12B family.

POST-TRANSLATIONAL MODIFICATION

Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.