IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3] (ET1701-39)
Catalog# ET1701-39
Rabbit Monoclonal to IL-1 beta
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
Lane 1: THP-1 whole cell lysate
Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3] (ET1701-39)
Immunogen
Synthetic peptide within c-terminal human il1 beta.
Host
Rabbit
Positive Control
THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate, A431, Hela, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ087-3
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
IL-1 beta
SYNONYMS
Catabolin antibody;H1 antibody;IL 1 antibody;IL 1 beta antibody;IL-1 beta antibody;IL1 BETA antibody;IL1B antibody;IL1B_HUMAN antibody;IL1F2 antibody;Interleukin 1 beta antibody;Interleukin-1 beta antibody;OAF antibody;OTTHUMP00000162031 antibody;Preinterleukin 1 beta antibody;Pro interleukin 1 beta antibody
SEQUENCE SIMILARITIES
Belongs to the IL-1 family.
TISSUE SPECIFICITY
Expressed in activated monocytes/macrophages (at protein level).
POST-TRANSLATIONAL MODIFICATION
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
SUBCELLULAR LOCATION
Cytoplasm, Lysosome, extracellular exosome, Secreted.
FUNCTION
Two forms of interleukin-1, designated IL-1α and IL-1β, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1α and IL-1β bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1β for production of the anti- pathogen IFN-γ. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.
CITATIONS
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Song S, Wang Q, Qu Y, et al
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WB
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Rat
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Lei, B., Liu, J., Yao, Z., Xiao, Y., Zhang, X., Zhang, Y., & Xu, J.
NF-κB-Induced Upregulation of miR-146a-5p Promoted Hippocampal Neuronal Oxidative Stress and Pyroptosis via TIGAR in a Model of Alzheimer's Disease. Frontiers in cellular neuroscience, 15, 653881.
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Human
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Human
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IF
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Human
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Feng Zhuan,et al
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Mouse
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Citation
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Li, Xin;Wang, Chunchun;Zhu, Jiang;Lin, Qian;Yu, Minjie;Wen, Jiashu;Feng, Jie;Hu, Caihong
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Pig
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IHC
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Mouse
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VEGF-Modified PVA/Silicone Nanofibers Enhance Islet Function Transplanted in Subcutaneous Site Followed by Device-Less Procedure
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IHC
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Mouse
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Mi Ai,et al
Hepatoprotective efficacy and interventional mechanism of panaxanediol saponins component in high-fat diet-induced NAFLD mice
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WB
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Mouse
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Citation
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Curcumin attenuates MSU crystal-induced inflammation by inhibiting the degradation of IκBα and blocking mitochondrial damage.
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WB
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Human
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Citation
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Huang Xiuhong,Fu Meimei,Lu Min,et al
Polyphenol-Based Photothermal Nanoparticles with Sprayable Capability for Self-regulation of Microenvironment to Accelerate Diabetic Wound Healing
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IHC
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IF
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Human
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Citation
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