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PRODUCT CODE: R1511-15

IL-8 Rabbit Polyclonal Antibody (R1511-15)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of IL-8 on human kidney tissue lysate using anti-IL-8 antibody at 1/1,000 dilution.
  • Western blot analysis of IL-8 on human kidney tissue lysate using anti-IL-8 antibody at 1/1,000 dilution.
  • ICC staining IL-8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of HUVEC cells with IL-8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of IL-8 on human kidney tissue lysate using anti-IL-8 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL-8 Rabbit Polyclonal Antibody (R1511-15)

Immunogen

Recombinant protein

Host

Rabbit

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

Isotype

IgG

TARGET

UNIPROT #

P10145

PROTEIN NAME

IL-8

SEQUENCE SIMILARITIES

Belongs to the intercrine alpha (chemokine CxC) family.

POST-TRANSLATIONAL MODIFICATION

Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.; Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.