IL-8 Rabbit Polyclonal Antibody (R1511-15)
Catalog# R1511-15
Rabbit Polyclonal to IL-8
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WB
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IHC-P
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IF-Cell
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FC
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Human
Lane 1: U-87 MG cell lysate
Lane 2: U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
IL-8 Rabbit Polyclonal Antibody (R1511-15)
Immunogen
Recombinant protein of human il-8 aa 1-99 / 99.
Host
Rabbit
Positive Control
U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate, 293T, HUVEC.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:50
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FC
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 11 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
IL-8
SYNONYMS
(Ala-IL-8)77 antibody;(Ser-IL-8)72 antibody;9E3 antibody;Beta thromboglobulin like protein antibody;C-X-C motif chemokine 8 antibody;CEF-4 antibody;chemokine, CXC motif, ligand 8 antibody;CXCL8 antibody;Emoctakin antibody;GCP-1 antibody;GCP/IL-8 protein I antibody;GCP/IL-8 protein II antibody;GCP/IL-8 protein III antibody;GCP/IL-8 protein IV antibody;GCP/IL-8 protein V antibody;GCP/IL-8 protein VI antibody;GCP1 antibody;Granulocyte chemotactic protein 1 antibody;IL-8 antibody;IL-8(1-77) antibody;IL-8(9-77) antibody;IL8 antibody;IL8/NAP1 form I antibody;IL8/NAP1 form II antibody;IL8/NAP1 form III antibody;IL8/NAP1 form IV antibody;IL8/NAP1 form V antibody;IL8/NAP1 form VI antibody;IL8_HUMAN antibody;Inteleukin 8 antibody;LECT antibody;LUCT antibody;Lymphocyte-derived neutrophil-activating factor antibody;LYNAP antibody;MDNCF antibody;MDNCF-b antibody;MDNCF-c antibody;MONAP antibody;Monocyte derived neutrophil activating peptide antibody;Monocyte derived neutrophil chemotactic factor antibody;Monocyte-derived neutrophil chemotactic factor antibody;Monocyte-derived neutrophil-activating peptide antibody;NAF antibody;NAP 1 antibody;NAP-1 antibody;NAP1 antibody;Neutrophil activating peptide 1 antibody;Neutrophil activating protein 1 antibody;Neutrophil-activating factor antibody;Neutrophil-activating protein 1 antibody;Protein 3 10C antibody;Protein 3-10C antibody;SCYB 8 antibody;SCYB8 antibody;Small inducible cytokine subfamily B member 8 antibody;T cell chemotactic factor antibody;T-cell chemotactic factor antibody;TSG 1 antibody;TSG1 antibody
SEQUENCE SIMILARITIES
Belongs to the intercrine alpha (chemokine CxC) family.
POST-TRANSLATIONAL MODIFICATION
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.; Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.
SUBCELLULAR LOCATION
Secreted
FUNCTION
IL-8, also known as neutrophil chemotactic factor, has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also stimulates phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis (e.g. histamine release), and the respiratory burst.IL-8 can be secreted by any cells with toll-like receptors that are involved in the innate immune response. Usually, it is the macrophages that see an antigen first, and thus are the first cells to release IL-8 to recruit other cells. Both monomer and homodimer forms of IL-8 have been reported to be potent inducers of the chemokine receptors CXCR1 and CXCR2. The homodimer is more potent, but methylation of Leu25 can block the activity of homodimers.
CITATIONS
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Yin, X., Teng, X., Ma, T., Yang, T., Zhang, J., Huo, M., Liu, W., Yang, Y., Yuan, B., Yu, H., Huang, W., & Wang, Y.
RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis
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WB
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Human
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Citation
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