PRODUCT CODE: ER1706-67

IL-8 Rabbit Polyclonal Antibody (ER1706-67)

Applications

  • ELISA

  • ICC

  • IHC-P

REACTIVITY

  • Human

ICC staining IL-8 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IL-8 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IL-8 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IL-8 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IL-8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-8 antibody. Counter stained with hematoxylin.
ICC staining IL-8 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ELISA

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL-8 Rabbit Polyclonal Antibody (ER1706-67)

Immunogen

Synthetic peptide within c-terminal human il-8.

Host

Rabbit

Positive Control

HUVEC, PANC-1, PC-3M, human tonsil tissue, human kidney tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:200-1:1,000

  • IHC-P

  • 1:100-1:500

  • ELISA

  • 1:5,000-1:10,000

TARGET

UNIPROT #

PROTEIN NAME

IL-8

SYNONYMS

(Ala-IL-8)77 antibody; (Ser-IL-8)72 antibody; 9E3 antibody; Beta thromboglobulin like protein antibody; C-X-C motif chemokine 8 antibody; CEF-4 antibody; chemokine, CXC motif, ligand 8 antibody; CXCL8 antibody; Emoctakin antibody; GCP-1 antibody; GCP/IL-8 protein I antibody; GCP/IL-8 protein II antibody; GCP/IL-8 protein III antibody; GCP/IL-8 protein IV antibody; GCP/IL-8 protein V antibody; GCP/IL-8 protein VI antibody; GCP1 antibody; Granulocyte chemotactic protein 1 antibody; IL-8 antibody; IL-8(1-77) antibody; IL-8(9-77) antibody; IL8 antibody; IL8/NAP1 form I antibody; IL8/NAP1 form II antibody; IL8/NAP1 form III antibody; IL8/NAP1 form IV antibody; IL8/NAP1 form V antibody; IL8/NAP1 form VI antibody; IL8_HUMAN antibody; Inteleukin 8 antibody; LECT antibody; LUCT antibody; Lymphocyte-derived neutrophil-activating factor antibody; LYNAP antibody; MDNCF antibody; MDNCF-b antibody; MDNCF-c antibody; MONAP antibody; Monocyte-derived neutrophil chemotactic factor antibody; Monocyte-derived neutrophil-activating peptide antibody; NAF antibody; NAP-1 antibody; NAP1 antibody; Neutrophil activating peptide 1 antibody; Neutrophil activating protein 1 antibody; Neutrophil-activating factor antibody; Neutrophil-activating protein 1 antibody; Protein 3 10C antibody; Protein 3-10C antibody; SCYB8 antibody; Small inducible cytokine subfamily B member 8 antibody; T cell chemotactic factor antibody; T-cell chemotactic factor antibody; TSG1 antibody

SEQUENCE SIMILARITIES

Belongs to the intercrine alpha (chemokine CxC) family.

POST-TRANSLATIONAL MODIFICATION

Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.; Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.