PRODUCT CODE: ER1706-68

IL-7R-alpha Rabbit Polyclonal Antibody (ER1706-68)

  • Zebrafish

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis of IL-7R-alpha on zebrafish tissue lysate using anti-IL-7R-alpha antibody at 1/500 dilution.
  • Western blot analysis of IL-7R-alpha on zebrafish tissue lysate using anti-IL-7R-alpha antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-IL-7R-alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Daudi cells with IL-7R-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of IL-7R-alpha on zebrafish tissue lysate using anti-IL-7R-alpha antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL-7R-alpha Rabbit Polyclonal Antibody (ER1706-68)

Immunogen

Recombinant protein within human il-7r-alpha aa 222-392.

Host

Rabbit

Positive Control

Zebrafish, Daudi.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

IL-7R-alpha

SYNONYMS

CD 127 antibody; CD127 antibody; CD127 antigen antibody; CDw127 antibody; IL 7R alpha antibody; IL 7R antibody; IL-7 receptor subunit alpha antibody; IL-7R subunit alpha antibody; IL-7R-alpha antibody; IL-7RA antibody; IL7R antibody; IL7RA antibody; IL7RA_HUMAN antibody; IL7Ralpha antibody; ILRA antibody; Interleukin 7 receptor alpha chain antibody; Interleukin 7 receptor antibody; Interleukin 7 receptor isoform H5 6 antibody; Interleukin-7 receptor subunit alpha antibody

SEQUENCE SIMILARITIES

Belongs to the type I cytokine receptor family. Type 4 subfamily.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.

SUBCELLULAR LOCATION

Membrane. Secreted.

FUNCTION

Interleukin 7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor-specific chain and the IL-2 receptor g chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2), originally called 4PS.