IL-6R Rabbit Polyclonal Antibody (HA500438)
Catalog# HA500438
Rabbit Polyclonal to IL-6R
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Western blot analysis of IL-6R on different lysates with Rabbit anti-IL-6R antibody (HA500438) at 1/1,000 dilution.
Lane 1: Daudi cell lysate
Lane 2: A549 cell lysate
Lane 3: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 47 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500438) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Daudi cell lysate
Lane 2: A549 cell lysate
Lane 3: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 47 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500438) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.