PRODUCT CODE: EM30301

IL-6 Mouse Monoclonal Antibody [7-A7] (EM30301)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of IL-6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM30301, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: Raji cell lysate<br />
Lane 3: Daudi cell lysate
  • Western blot analysis of IL-6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM30301, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: Raji cell lysate<br />
Lane 3: Daudi cell lysate
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM30301, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of IL-6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM30301, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Raji cell lysate
Lane 3: Daudi cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

IL-6 Mouse Monoclonal Antibody [7-A7] (EM30301)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Jurkat, Raji, Daudi, human tonsil tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7-A7

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

IL-6

SYNONYMS

Interleukin BSF 2 antibody; B cell differentiation factor antibody; B cell stimulatory factor 2 antibody; B-cell stimulatory factor 2 antibody; BSF 2 antibody; BSF-2 antibody; BSF2 antibody; CDF antibody; CTL differentiation factor antibody; Hepatocyte stimulatory factor antibody; HGF antibody; HSF antibody; Hybridoma growth factor antibody; Hybridoma growth factor Interferon beta-2 antibody; IFN-beta-2 antibody; IFNB2 antibody; IL 6 antibody; IL-6 antibody; IL6 antibody; IL6_HUMAN antibody; Interferon beta 2 antibody; Interferon beta-2 antibody; Interleukin 6 antibody; Interleukin 6 (interferon beta 2) antibody; Interleukin BSF 2 antibody; Interleukin-6 antibody

SEQUENCE SIMILARITIES

Belongs to the IL-6 superfamily.

SUBCELLULAR LOCATION

Secreted

FUNCTION

Interleukin 6 (IL-6) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL-6 is a cytokine with a wide variety of biological functions and belongs to the?IL-6 superfamily. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation.