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PRODUCT CODE: EM1701-79

IL-31 Mouse Monoclonal Antibody [8-C3] (EM1701-79)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

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Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.
  • Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.
  • ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-31 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-31 antibody. Counter stained with hematoxylin.
Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

IL-31 Mouse Monoclonal Antibody [8-C3] (EM1701-79)

Immunogen

Recombinant full length protein corresponding to human il18.

Host

Mouse

Positive Control

Recombinant protein lysate, A431, LOVO, MCF-7, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

8-C3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

18 kDa

Isotype

IgG3

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

Q6EBC2

PROTEIN NAME

IL-31

SYNONYMS

IL 31 antibody; IL-31 antibody; IL31 antibody; IL31_HUMAN antibody; Interleukin 31 antibody; Interleukin-31 antibody

TISSUE SPECIFICITY

Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

IL-31 is a T cell cytokine that is preferentially produced by T helper type 2 cells. IL-31 signals through a heterodimeric receptor composed of the IL-31 receptor (IL-31R) and the oncostatin M receptor (OSM). This receptor complex recruits JAK1, JAK2, Stat1, Stat3 and Stat5 signaling pathways, as well as the PI3 kinase/AKT cascade. SHP-2 and Shc adapter molecules are also recruited and contribute to an increased activation of the MAP kinase pathway in response to IL-31. Overexpression of IL-31 in mice results in pruritus and skin dermatitis resembling human atopic dermatitis (AD). Comparisons between skin from patients with AD and healthy skin showed IL-31R expression at higher levels on epidermal keratinocytes in AD samples. Infiltrating cells, more numerous in skin from patients with AD compared with that of healthy individuals, expressed IL-31 mRNA. IL-31 may participate in the cause of itch sensation and promote scratching behavior in NC/Nga mice with atopic dermatitis, and may represent a novel target for antipruritic drug development.