PRODUCT CODE: ER1706-91

IL-17 Rabbit Polyclonal Antibody (ER1706-91)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of IL-17 on human fetal skeletal muscle tissue lysates using anti-IL-17 antibody at 1/500 dilution.
  • Western blot analysis of IL-17 on human fetal skeletal muscle tissue lysates using anti-IL-17 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-IL-17 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-IL-17 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-IL-17 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with IL-17 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of IL-17 on human fetal skeletal muscle tissue lysates using anti-IL-17 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL-17 Rabbit Polyclonal Antibody (ER1706-91)

Immunogen

Synthetic peptide within human il-17 aa 60-109 / 155.

Host

Rabbit

Positive Control

Rat skeletal muscle tissue, rat heart tissue, human tonsil tissue, human fetal skeletal muscle tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

Predicted band size 17.5 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

IL-17

SYNONYMS

CTLA 8 antibody; CTLA-8 antibody; CTLA8 antibody; Cytotoxic T lymphocyte associated antigen 8 antibody; Cytotoxic T lymphocyte associated protein 8 antibody; Cytotoxic T lymphocyte associated serine esterase 8 antibody; Cytotoxic T-lymphocyte-associated antigen 8 antibody; IL 17A antibody; IL-17 antibody; IL-17A antibody; IL17 antibody; IL17_HUMAN antibody; Il17a antibody; Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody; Interleukin 17A antibody; Interleukin-17A antibody; Interleukin17 antibody; Interleukin17A antibody; OTTHUMP00000016597 antibody; OTTMUSP00000046003 antibody

SEQUENCE SIMILARITIES

Belongs to the IL-17 family.

TISSUE SPECIFICITY

Restricted to activated memory T-cells.

POST-TRANSLATIONAL MODIFICATION

Found both in glycosylated and nonglycosylated forms.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Cytokines are small, soluble proteins with pleiotropic effects on a variety of cell types. Cytokines have a regulatory function over the immune system and mediate aspects of inflammatory response. They exert their biological effects through the binding of membrane-bound receptors which, in turn, initiate signal transduction cascades and elicit physiological changes in their target cell. Interleukin-17 (IL-17) and its cognate receptor, IL-17R, are an example of such a cytokine receptor pair. Originally identified as a rodent cDNA termed CTLA8, IL-17 is capable of inducing the secretion of IL-6 and IL-8 and augmenting the expression of ICAM-1 in human fibroblast cultures. The IL-17 protein exhibits a striking degree of homology with the HSV13 protein which mimics its function. The IL-17 receptor is a type I transmembrane protein 864 amino acids in length, that is highly expressed in spleen and kidney.