IL-13R alpha 2 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-85] (HA750910)
Catalog# HA750910
Rabbit Monoclonal to IL-13R alpha 2
Tested for
-
WB
-
IF-Cell
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IHC-P
-
FC
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody (HA750910) at 1/2,000 dilution.
Lane 1: A375 cell lysate (no heat)
Lane 2: U-937 cell lysate (no heat) (negative)
Lane 3: Daudi cell lysate (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 50-80 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750910) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody (HA750910) at 1/2,000 dilution.
Lane 1: A375 cell lysate (no heat)
Lane 2: U-937 cell lysate (no heat) (negative)
Lane 3: Daudi cell lysate (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 50-80 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750910) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.