IL-1 beta Mouse Monoclonal Antibody [A7A10] (HA601035)
Catalog# HA601035
Mouse Monoclonal to IL-1 beta
Tested for
-
WB
-
ELISA
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IF-Cell
Reacts with
-
Human
Western blot analysis of IL-1 beta on different lysates with Mouse anti-IL-1 beta antibody (HA601035) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601035) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601035) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.