PRODUCT CODE: ER30911

Ikk alpha Rabbit Polyclonal Antibody (ER30911)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IKK alpha on different cell lysates using anti-IKK alpha antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: Hela           <br />
Lane 2: HepG2 <br />
Lane 3: Raji           <br />
Lane 4: NIH/3T3 <br />
Lane 5: PC12           <br />
Lane 6: Jurkat
  • Western blot analysis of IKK alpha on different cell lysates using anti-IKK alpha antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: Hela           <br />
Lane 2: HepG2 <br />
Lane 3: Raji           <br />
Lane 4: NIH/3T3 <br />
Lane 5: PC12           <br />
Lane 6: Jurkat
  • ICC staining IKK alpha in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human mammary gland tissue using anti-IKK alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-IKK alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with IKK alpha antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of IKK alpha on different cell lysates using anti-IKK alpha antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: Raji
Lane 4: NIH/3T3
Lane 5: PC12
Lane 6: Jurkat

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Ikk alpha Rabbit Polyclonal Antibody (ER30911)

Immunogen

Synthetic peptide within n-terminal human ikk alpha.

Host

Rabbit

Positive Control

Hela, NIH/3T3, HepG2, Raji, PC12, N2A, Jurkat, human mammary gland tissue, human gastric carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

85 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ikk alpha

SYNONYMS

chuk antibody; CHUK1 antibody; Conserved Helix Loop Helix Ubiquitous Kinase antibody; Conserved helix loop ubiquitous kinase antibody; Conserved helix-loop-helix ubiquitous kinase antibody; I Kappa B Kinase 1 antibody; I Kappa B Kinase Alpha antibody; I-kappa-B kinase 1 antibody; I-kappa-B kinase alpha antibody; IkappaB kinase antibody; IkB kinase alpha subunit antibody; IkBKA antibody; IKK 1 antibody; IKK A antibody; IKK a kinase antibody; IKK-A antibody; IKK-alpha antibody; IKK1 antibody; IKKA antibody; IKKA_HUMAN antibody; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody; Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody; NFKBIKA antibody; Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody; Nuclear factor NF kappa B inhibitor kinase alpha antibody; Nuclear factor NF-kappa-B inhibitor kinase alpha antibody; Nuclear factor NFkappaB inhibitor kinase alpha antibody; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody; TCF-16 antibody; TCF16 antibody; Transcription factor 16 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.; (Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

SUBCELLULAR LOCATION

Cytoplasm, nucleus.

FUNCTION

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis.