Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of IgG4 on different lysates with Rabbit anti-IgG4 antibody (ET1609-56) at 1/500 dilution.
Lane 1: human serum lysate
Lane 2: human plasma lysate
Lane 2: human spleen lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-56) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Several studies have shown that a large population of patients with recurrent respiratory tract infection have low IgG4 concentrations.