PRODUCT CODE: ET1701-88

IGF2 Recombinant Rabbit Monoclonal Antibody [JJ092-3] (ET1701-88)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of IGF2 on human placenta lysates using anti-IGF2 antibody at 1/1,000 dilution.
  • Western blot analysis of IGF2 on human placenta lysates using anti-IGF2 antibody at 1/1,000 dilution.
  • ICC staining IGF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IGF2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IGF2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IGF2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IGF2 antibody. Counter stained with hematoxylin.
Western blot analysis of IGF2 on human placenta lysates using anti-IGF2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

IGF2 Recombinant Rabbit Monoclonal Antibody [JJ092-3] (ET1701-88)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293, 293T, Hela, HepG2, human liver cancer tissue, human colon cancer tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ092-3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

IGF2

SYNONYMS

C11orf43 antibody; IGF 2 antibody; IGF II antibody; IGF-II antibody; IGF2 antibody; IGF2_HUMAN antibody; IGFII antibody; INSIGF antibody; insulin like growth factor 2 (somatomedin A) antibody; Insulin like Growth Factor 2 antibody; Insulin like growth factor II antibody; Insulin like growth factor II precursor antibody; Insulin like growth factor type 2 antibody; pp9974 antibody; Preptin antibody; putative insulin like growth factor II associated protein antibody; Somatomedin A antibody; Somatomedin-A antibody

SEQUENCE SIMILARITIES

Belongs to the insulin family.

TISSUE SPECIFICITY

Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.

DEVELOPMENTAL STAGE

During embryogenesis, detected in liver, lung, skeletal muscle and placenta.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99.; Proteolytically processed by PCSK4, proIGF2 is cleaved at Arg-128 and Arg-92 to generate big-IGF2 and mature IGF2.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors. It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation. In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter. This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent.