Applications
-
WB
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
IGF1R Mouse Monoclonal Antibody [D5-G2] (HA600033)
Immunogen
Recombinant protein within human igf1r aa 600-1000.
Host
Mouse
Positive Control
293 cell lysates, SiHa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
D5-G2
PROPERTIES
Form
Liquid
Storage Condition
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
MOLECULAR WEIGHT
Predicted band size: 155 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:500
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
IGF1R
SYNONYMS
Insulin-like growth factor 1 receptor Insulin-like growth factor I receptor IGF-I receptor CD221 Insulin-like growth factor 1 receptor alpha chain Insulin-like growth factor 1 receptor beta chain
SUBCELLULAR LOCATION
Cell membrane, Membrane.
FUNCTION
Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis.