PRODUCT CODE: R1511-30

IFNAR1 Rabbit Polyclonal Antibody (R1511-30)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

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ICC staining of IFNAR1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1511-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of IFNAR1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1511-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of IFNAR1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1511-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ICC staining of IFNAR1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1511-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IFNAR1 Rabbit Polyclonal Antibody (R1511-30)

Immunogen

Peptide

Host

Rabbit

Positive Control

SW620, K562.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

Isotype

IgG

TARGET

UNIPROT #

PROTEIN NAME

IFNAR1

SYNONYMS

Alpha type antiviral protein antibody; Antiviral protein, alpha-type antibody; Antiviral protein, beta-type antibody; AVP antibody; Beta type antiviral protein antibody; CRF2-1 antibody; Cytokine receptor class-II member 1 antibody; Cytokine receptor family 2 member 1 antibody; IFN alpha REC antibody; IFN alpha receptor antibody; IFN alpha/beta Receptor alpha antibody; IFN beta receptor antibody; IFN Interferon-beta receptor antibody; IFN-alpha/beta receptor 1 antibody; IFN-R-1 antibody; IFNAR antibody; Ifnar1 antibody; IFNBR antibody; IFRC antibody; INAR1_HUMAN antibody; Interferon (alpha beta and omega) receptor 1 antibody; interferon alpha and beta receptor subunit 1 antibody; Interferon alpha/beta receptor 1 antibody; Interferon alpha/beta receptor alpha chain antibody; Interferon beta receptor 1 antibody; interferon receptor 1 antibody; Interferon-alpha receptor antibody; Type I interferon receptor 1 antibody

SEQUENCE SIMILARITIES

Belongs to the type II cytokine receptor family.

TISSUE SPECIFICITY

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex.; Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination. Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Phosphorylated on tyrosine residues in response to interferon.; Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.

SUBCELLULAR LOCATION

Late endosome, Lysosome, Cell membrane.

FUNCTION

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.