PRODUCT CODE: ER1802-4

Ifna1 Rabbit Polyclonal Antibody (ER1802-4)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
  • Western blot analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
  • ICC staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with IFNA1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Ifna1 Rabbit Polyclonal Antibody (ER1802-4)

Immunogen

Synthetic peptide within mouse ifna1 aa 140-181.

Host

Rabbit

Positive Control

Mouse liver tissue lysate, N2A, SHG-44, SH-SY5Y, rat brain tissue, human kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Ifna1

SYNONYMS

IFN-alpha-14 antibody; IFN14_HUMAN antibody; IFNA14 antibody; Interferon alpha 14 antibody; Interferon alpha H antibody; Interferon alpha-14 antibody; Interferon alpha-H antibody; Interferon lambda-2-H antibody; Interferon lambda2H antibody; LeIF H antibody; LeIFH antibody; MGC125756 antibody; MGC125757 antibody

SEQUENCE SIMILARITIES

Belongs to the alpha/beta interferon family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

The genes encoding type I interferons (IFNs), which include 14 IFN-α genes (two of which are IFN-α1 and IFN-α13), one IFN-β gene, one IFN-w (also known as IFN-α II1) gene and a number of IFN-w pseudogenes, are clustered on human chromosome 9. IFN-α and -β are cytokines that are widely known to induce potent antiviral activity. They exert a variety of other biological effects, including antitumor and immunomodulatory activities and are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. IFN-w is antigenically different from human IFN-α, IFN-β or IFN-γ, but is a component of natural mixtures of IFN species produced by virus-induced leukocytes or Burkitt's lymphoma cells. The type I interferon receptor (IFN-αR) interacts with IFN-α, IFN-β and IFN-w, and seems to be a multisubunit receptor.