iFluor™ 647 Conjugated Goat anti-mouse IgG Goat Polyclonal Antibody (HA1127)
Catalog# HA1127
Goat Polyclonal to Goat anti-mouse IgG
-
IF-Cell
-
IF-Tissue
-
IHC-Fr
-
Mouse
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta Ⅲ tubulin antibody (M0805-8) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Specifications
Product Type
Goat polyclonal secondary
Product Name
iFluor™ 647 Conjugated Goat anti-mouse IgG Goat Polyclonal Antibody (HA1127)
Immunogen
Mouse igg (h+l).
Specificity
To minimize cross-reactivity, the ifluor™ 647 conjugated goat anti-mouse igg (h+l) antibodies have been highly cross-adsorbed against human igg.
Host
Goat
Conjugation
Ifluor™ 647
Clonality
Polyclonal
APPLICATION DILUTION
-
IF-Cell
-
1:500-1:1,000
-
IF-Tissue
-
1:500-1:1,000
-
IHC-Fr
-
1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
Goat anti-mouse IgG
FUNCTION
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
CITATIONS
-
Ma Zhixin,et al
Extracellular vesicles containing MFGE8 from colorectal cancer facilitate macrophage efferocytosis
-
IF
-
Citation
-
-
Rui Guo,et al
A biomimetic solution, albumin–doxorubicin molecular complex, targeting tumor and tumor-draining lymph nodes
-
IHC
-
Mouse
-
Citation
-
-
Expression and functional identification of recombinant SARS-CoV-2 receptor binding domain (RBD) from E. coli system
-
FC
-
Mouse
-
Citation
-
-
RNA-Editing Enzyme ADAR1 p150 Isoform Is Critical for Germinal Center B Cell Response
-
IF-cell
-
Human
-
Citation
-
-
Gao Yangshuai,et al
Nr4a1 enhances Wnt4 transcription to promote mesenchymal stem cell osteogenesis and alleviates inflammation-inhibited bone regeneration
-
IF
-
Mouse
-
Citation
-
-
An efficient system to generate truncated human angiotensin converting enzyme 2 (hACE2) capable of binding RBD and spike protein of SARS-CoV2
-
FC
-
Human
-
Citation
-
-
Sun Y, Xiao W, Yu Y, et al
Colorectal cancer-derived extracellular vesicles containing HSP70 enhance macrophage phagocytosis by up-regulating MARCO expression
-
IF
-
Mouse
-
Citation
-
