iFluor™ 594 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1122)
Catalog# HA1122
Goat Polyclonal to Goat anti-rabbit IgG
Tested for
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ICC
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IF
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FC
React with
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Rabbit
Conjugated to iFluor™ 594
Immunocytochemistry analysis of SKOV-3 cells labeling Vimentin (ET1610-39).
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Vimentin (ET1610-39, red) at 1/200 dilution for overnight at 4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/800 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Vimentin (ET1610-39, red) at 1/200 dilution for overnight at 4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/800 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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