iFluor™ 594 Conjugated Goat anti-mouse IgG Goat Polyclonal Antibody (HA1126)
Catalog# HA1126
Goat Polyclonal to Goat anti-mouse IgG
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IF-Cell
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IF-Tissue
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FC
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IHC-Fr
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Mouse
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies GFAP (EM140707, red) at 1/500 dilution and NeuN (ET1602-12, green) at 1/50 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) and Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.
Specifications
Product Type
Goat polyclonal secondary
Product Name
iFluor™ 594 Conjugated Goat anti-mouse IgG Goat Polyclonal Antibody (HA1126)
Immunogen
Mouse igg (h+l).
Specificity
To minimize cross-reactivity, the ifluor™ 594 conjugated goat anti-mouse igg (h+l) antibodies have been highly cross-adsorbed against human igg.
Host
Goat
Conjugation
Ifluor™ 594
Clonality
Polyclonal
APPLICATION DILUTION
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:1,000
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FC
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1:500-1:1,000
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IHC-Fr
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
Goat anti-mouse IgG
FUNCTION
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
CITATIONS
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Jianping Liu,et al
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IF
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Mouse
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Citation
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IF
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Mouse
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IF-cell
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Human
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IF
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Human
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Citation
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IHC;IF
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IF
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Mouse
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IF
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Citation
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Xin Huang,et al
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IF
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Citation
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Anemoside B4 Exerts Hypoglycemic Effect by Regulating the Expression of GLUT4 in HFD/STZ Rats
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IF-tissue
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rat
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Citation
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Leverage of nuclease-deficient CasX for preventing pathological angiogenesis
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ICC
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Mouse
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Citation
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Song Mingrui,et al
Granulocyte colony-stimulating factor mediates bone loss via the activation of IL-1β/JNK signaling pathway in murine Staphylococcus aureus-induced osteomyelitis
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IF
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Citation
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Hu Qin,et al
Capsaicin Attenuates LPS-Induced Acute Lung Injury by Inhibiting Inflammation and Autophagy Through Regulation of the TRPV1/AKT Pathway
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IF
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Human
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Citation
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