iFluor™ 594 Conjugated Goat anti-mouse IgG Goat Polyclonal Antibody (HA1126)
Catalog# HA1126
Goat Polyclonal to Goat anti-mouse IgG
Tested for
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ICC
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IF
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FC
React with
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Mouse
Conjugated to iFluor™ 594
Immunocytochemistry analysis of Hela cells labeling beta Ⅲ tubulin (M0805-8).
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody beta Ⅲ tubulin (M0805-8, red) at 1/100 dilution for overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/800 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody beta Ⅲ tubulin (M0805-8, red) at 1/100 dilution for overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/800 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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