iFluor™ 594 Conjugated Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04] (HA720121F)
Catalog# HA720121F
Rabbit Monoclonal to Cytokeratin 5
Tested for
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IF-Tissue
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 594
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720121F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720121F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720121F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).