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iFluor™ 488 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1121)

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Catalog# HA1121

Goat Polyclonal to Goat anti-rabbit IgG

Tested for

  • IF-Cell

  • IF-Tissue

  • FC

  • IHC-Fr

Reacts with

  • Rabbit

Conjugated to iFluor™ 488
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Goat polyclonal secondary

Product Name

iFluor™ 488 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1121)

Immunogen

Rabbit igg (h+l).

Specificity

To minimize cross-reactivity, the ifluor™ 488 conjugated goat anti-rabbit igg (h+l) antibodies have been highly cross-adsorbed against human igg and mouse igg.

Host

Goat

Conjugation

Ifluor™ 488

Clonality

Polyclonal

APPLICATION DILUTION

  • IF-Cell

  • 1:500-1:1,000

  • IF-Tissue

  • 1:500-1:1,000

  • FC

  • 1:500-1:1,000

  • IHC-Fr

  • 1:500-1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Concentration

2ug/ul

Purification

Immunogen affinity purified.

Isotype

IgG

TARGET

PROTEIN NAME

Goat anti-rabbit IgG

FUNCTION

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.

CITATIONS

  • Gong, Y., Qiu, J., Ye, J., Jiang, T., Zhang, W., Zheng, X., Zhu, Z., Chen, L., Wang, Z., Mi, S., & Hong, Z.

    AZ-628 delays osteoarthritis progression via inhibiting the TNF-α-induced chondrocyte necroptosis and regulating osteoclast formation

  • Ze Li,et al

    Tomatidine Alleviates Intervertebral Disc Degeneration by Activating the Nrf2/HO-1/GPX4 Signaling Pathway

  • Xing Yien,et al

    Comparison of the immune response and protection against the experimental Toxoplasma gondii infection elicited by immunization with the recombinant proteins BAG1, ROP8, and BAG1-ROP8

  • Peng Zhiwei,et al

    Crosstalk between cancer-associated fibroblasts and myeloid cells shape the heterogeneous microenvironment of gastric cancer

  • Wenjing Zeng,et al

    SENP1 prevents high fat diet-induced non-alcoholic fatty liver diseases by regulating mitochondrial dynamics

  • Zhu Zhaoying,et al

    IKIP downregulates THBS1/FAK signaling to suppress migration and invasion by glioblastoma cells

  • PD0325901, an ERK inhibitor, attenuates RANKL-induced osteoclast formation and mitigates cartilage inflammation by inhibiting the NF-κB and MAPK pathways

  • Jianping Liu,et al

    Semaglutide alleviates the pancreatic β cell function via the METTL14 signaling and modulating gut microbiota in type 2 diabetes mellitus mice

  • Ye, S., Cao, Q., Ni, P., Xiong, S., Zhong, M., Yuan, T., Shan, J., Liang, J., Fan, Y., & Zhang, X.

    Construction of Microfluidic Chip Structure for Cell Migration Studies in Bioactive Ceramics

  • Chengwei Wu,et al

    NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer

  • Liang, M., Guo, X., Wu, H., & Zhong, Z.

    Inducible co-stimulator inhibits lipid phagocytosis of human aortic smooth muscle cells by down-regulating CD36 expression

  • Zhang Shuxia,et al

    CPEB1 Controls NRF2 Proteostasis and Ferroptosis Susceptibility in Pancreatic Cancer

  • Jia-Ming Chen,et al

    Transcriptomics of tissue exosomes to investigate miR-195-5p's amelioration of endometrial fibrosis via the YAP-Smad7 pathway: an animal study

  • Shan-Shan Chen,et al

    Mutation of the SUMOylation site of Aurora-B disrupts spindle formation and chromosome alignment in oocytes

  • Deng Huan,et al

    Matrix Stiffness Regulated Endoplasmic Reticulum Stress-mediated Apoptosis of Osteosarcoma Cell through Ras Signal Cascades

  • Li Yan,et al

    The ANGPTL4-HIF-1α loop: a critical regulator of renal interstitial fibrosis

  • Deng Huan,et al

    Effects of matrix viscoelasticity on cell–matrix interaction, actin cytoskeleton organization, and apoptosis of osteosarcoma MG-63 cells

  • Parthenolide alleviates microglia‐mediated neuroinflammation via MAPK/TRIM31/NLRP3 signaling to ameliorate cognitive disorder

  • Gong Y, Gao J, Li M, Zhang XL, Liao YH, Bao YB

    URP20 improves corneal injury caused by alkali burns combined with pathogenic bacterial infection in rats

  • Huifeng Xi,et al

    Diacetoxy-6-gingerdiol protects the extracellular matrix of nucleus pulposus cells and ameliorates intervertebral disc degeneration by inhibiting the IL-1β-mediated NLRP3 pathway.

  • Chi Xintian,et al

    A cross-sectional and longitudinal integrated study on brain functional changes in a neuropathic pain rat model

  • Bai Songjie,et al

    Extracellular vesicles from alveolar macrophages harboring phagocytosed methicillin-resistant Staphylococcus aureus induce necroptosis

  • Ling, Y. H., Wang, H., Han, M. Q., Wang, D., Hu, Y. X., Zhou, K., & Li, Y.

    Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein

  • Yongjing Guo,et al

    Maresin1 Inhibits Ferroptosis via the Nrf2/SLC7A11/GPX4 Pathway to Protect Against Sepsis-Induced Acute Liver Injury

  • Activation of the PERK-CHOP signaling pathway during endoplasmic reticulum stress contributes to olanzapine-induced dyslipidemia

  • Lu Weicheng,et al

    METTL14-mediated m6A epitranscriptomic modification contributes to chemotherapy-induced neuropathic pain by stabilizing GluN2A expression via IGF2BP2

  • α5-nAChR/STAT3/CD47 axis contributed to nicotine-related lung adenocarcinoma progression and immune escape

  • Zhou, H., Zhang, L., Luo, W., Hong, H., Tang, D., Zhou, D., Zhou, L., & Li, Y.

    AIM2 inflammasome activation benefits the therapeutic effect of BCG in bladder carcinoma

  • Hu Qin,et al

    Capsaicin Attenuates LPS-Induced Acute Lung Injury by Inhibiting Inflammation and Autophagy Through Regulation of the TRPV1/AKT Pathway

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