ICAM3 Recombinant Rabbit Monoclonal Antibody [JE63-07] (HA722467)
Catalog# HA722467
Rabbit Monoclonal to ICAM3
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ICAM3 on different lysates with Rabbit anti-ICAM3 antibody (HA722467) at 1/5,000 dilution.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 100-150 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722467) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 100-150 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722467) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.