PRODUCT CODE: ER131207

ICAM1 Rabbit Polyclonal Antibody (ER131207)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution.<br />
Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: HUVEC <br />
  Lane 3: K562
  • Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution.<br />
Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: HUVEC <br />
  Lane 3: K562
  • ICC staining of ICAM1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with ICAM1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution.
Positive control:
Lane 1: Raji
Lane 2: HUVEC
Lane 3: K562

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ICAM1 Rabbit Polyclonal Antibody (ER131207)

Immunogen

Synthetic peptide within human icam1 aa 483-532 / 532.

Host

Rabbit

Positive Control

Raji, HUVEC, K562, human tonsil tissue, Hela

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ICAM1

SYNONYMS

Antigen identified by monoclonal antibody; BB2 antibody; BB 2 antibody; BB2 antibody; CD 54 antibody; CD_antigen=CD54 antibody; CD54 antibody; Cell surface glycoprotein P3.58 antibody; Human rhinovirus receptor antibody; ICAM 1 antibody; ICAM-1 antibody; ICAM1 antibody; ICAM1_HUMAN antibody; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody; Intercellular adhesion molecule 1 antibody; Major group rhinovirus receptor antibody; MALA 2 antibody; MALA2 antibody; MyD 10 antibody; MyD10 antibody; P3.58 antibody; Surface antigen of activated B cells, BB2 antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. ICAM family.

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

SUBCELLULAR LOCATION

Cell membrane, Nucleus, Mitochondrion

FUNCTION

ICAM-1 is a member of the immunoglobulin superfamily, the superfamily of proteins including antibodies and T-cell receptors. The structure of ICAM-1 is characterized by heavy glycosylation, and the protein’s extracellular domain is composed of multiple loops created by disulfide bridges within the protein. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. More recently, ICAM-1 has been characterized as a site for the cellular entry of human rhinovirus. ICAM-1 and soluble ICAM-1 have antagonistic effects on the tight junctions forming the blood-testis barrier, thus playing a major role in spermatogenesis. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH).