ICAM1 Recombinant Rabbit Monoclonal Antibody [JE77-69] (HA722417)
Catalog# HA722417
Rabbit Monoclonal to ICAM1
Tested for
-
WB
-
IHC-P
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA722417) at 1/1,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse lung tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 100 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722417) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse lung tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 100 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722417) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.