PRODUCT CODE: M1505-7

ICAM1 Mouse Monoclonal Antibody [F11-A4] (M1505-7)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

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+
Western blot analysis on HUVEC cell lysates using anti-ICAM1 mouse mAb.
  • Western blot analysis on HUVEC cell lysates using anti-ICAM1 mouse mAb.
  • ICC staining ICAM1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis on HUVEC cell lysates using anti-ICAM1 mouse mAb.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ICAM1 Mouse Monoclonal Antibody [F11-A4] (M1505-7)

Immunogen

Peptide

Host

Mouse

Positive Control

HUVEC, HepG2

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

F11-A4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

90kDa

Isotype

IgG1a

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

ICAM1

SYNONYMS

Antigen identified by monoclonal antibody; BB2 antibody; BB 2 antibody; BB2 antibody; CD 54 antibody; CD_antigen=CD54 antibody; CD54 antibody; Cell surface glycoprotein P3.58 antibody; Human rhinovirus receptor antibody; ICAM 1 antibody; ICAM-1 antibody; ICAM1 antibody; ICAM1_HUMAN antibody; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody; Intercellular adhesion molecule 1 antibody; Major group rhinovirus receptor antibody; MALA 2 antibody; MALA2 antibody; MyD 10 antibody; MyD10 antibody; P3.58 antibody; Surface antigen of activated B cells, BB1 antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. ICAM family.

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

ICAM-1 is a member of the immunoglobulin superfamily, the superfamily of proteins including antibodies and T-cell receptors. The structure of ICAM-1 is characterized by heavy glycosylation, and the protein’s extracellular domain is composed of multiple loops created by disulfide bridges within the protein. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. More recently, ICAM-1 has been characterized as a site for the cellular entry of human rhinovirus. ICAM-1 and soluble ICAM-1 have antagonistic effects on the tight junctions forming the blood-testis barrier, thus playing a major role in spermatogenesis. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies.