PRODUCT CODE: ER1802-20

Iba1 Rabbit Polyclonal Antibody (ER1802-20)

Applications

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Iba1 antibody. Counter stained with hematoxylin.

Applications

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Iba1 Rabbit Polyclonal Antibody (ER1802-20)

Immunogen

Synthetic peptide within c-terminal human iba1.

Host

Rabbit

Positive Control

Human tonsil tissue, human spleen tissue, mouse spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

17/10/15 kDa

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Iba1

SYNONYMS

AIF 1 antibody; AIF-1 antibody; Aif1 antibody; AIF1 protein antibody; AIF1_HUMAN antibody; Allograft inflammatory factor 1 antibody; Allograft inflammatory factor 1 splice variant G antibody; allograft inflammatory factor-1 splice variant Hara-1 antibody; balloon angioplasty responsive transcription antibody; BART 1 antibody; G1 antibody; G1 putative splice variant of allograft inflamatory factor 1 antibody; IBA 1 antibody; IBA1 antibody; interferon gamma responsive transcript antibody; Interferon responsive transcript 1 antibody; interferon responsive transcript factor 1 antibody; Ionized calcium binding adapter molecule 1 antibody; Ionized calcium-binding adapter molecule 1 antibody; ionized calcium-binding adapter molecule antibody; IRT 1 antibody; IRT1 antibody; Microglia response factor antibody; MRF1 antibody; Protein g1 antibody

TISSUE SPECIFICITY

Detected in T-lymphocytes and peripheral blood mononuclear cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine residues.

SUBCELLULAR LOCATION

Plasma membrane. Cytoskeleton.

FUNCTION

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).