Iba1 Recombinant Mouse Monoclonal Antibody [8G8] (RT1316)
Catalog# RT1316
Mouse Monoclonal to Iba1
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WB
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IHC-P
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FC
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IP
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IHC-Fr
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
Iba1 Recombinant Mouse Monoclonal Antibody [8G8] (RT1316)
Immunogen
Recombinant protein.
Host
Mouse
Positive Control
Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, RAW264.7, C6, human PBL whole cell lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
8G8
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:500-1:2000
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IHC-Fr
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1:1,000
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IF-Cell
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1:200
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FC
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1:200-1:1,000
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IP
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Use at an assay dependent concentration
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IF-Tissue
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 17 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Iba1
SYNONYMS
AIF 1 antibody;AIF-1 antibody;Aif1 antibody;AIF1 protein antibody;AIF1_HUMAN antibody;Allograft inflammatory factor 1 antibody;Allograft inflammatory factor 1 splice variant G antibody;allograft inflammatory factor-1 splice variant Hara-1 antibody;balloon angioplasty responsive transcription antibody;BART 1 antibody;G1 antibody;G1 putative splice variant of allograft inflamatory factor 1 antibody;IBA 1 antibody;IBA1 antibody;interferon gamma responsive transcript antibody;Interferon responsive transcript 1 antibody;interferon responsive transcript factor 1 antibody;Ionized calcium binding adapter molecule 1 antibody;Ionized calcium-binding adapter molecule 1 antibody;ionized calcium-binding adapter molecule antibody;IRT 1 antibody;IRT1 antibody;Microglia response factor antibody;MRF1 antibody;Protein g1 antibody
SUBCELLULAR LOCATION
Cytoplasm, cytoskeleton.
FUNCTION
Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).
CITATIONS
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Shan, Z., Wang, X., Zong, W., Li, J., Zheng, B., Huang, B., Zhang, X., Chen, J., & Huang, Y.
The Effect of Cutibacterium acnes Infection on Nerve Penetration in the Annulus Fibrosus of Lumbar Intervertebral Discs via Suppressing Oxidative Stress
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IF
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Rat
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Citation
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Tao Xu;Aizhong Wang
Spinal TLR4/P2X7 Receptor-Dependent NLRP3 Inflammasome Activation Contributes to the Development of Tolerance to Morphine-Induced Antinociception
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IF
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Mouse
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Citation
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