HUWE1 Recombinant Rabbit Monoclonal Antibody [PSH14-37] (HA723631)
Catalog# HA723631
Rabbit Monoclonal to HUWE1
Tested for
-
WB
-
IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (HA723631) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SW480 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (low expression) (20 µg/Lane)
Lane 4: MOLT-4 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (20 µg/Lane)
Lane 8: F9 cell lysate (20 µg/Lane)
Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Predicted band size: 482 kDa
Observed band size: 482 kDa
Exposure time: 25 seconds; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723631) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (HA723631) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SW480 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (low expression) (20 µg/Lane)
Lane 4: MOLT-4 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (20 µg/Lane)
Lane 8: F9 cell lysate (20 µg/Lane)
Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Predicted band size: 482 kDa
Observed band size: 482 kDa
Exposure time: 25 seconds; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723631) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.