hUPF1 Recombinant Rabbit Monoclonal Antibody [JG38-44] (ET7108-70)
Catalog# ET7108-70
Rabbit Monoclonal to hUPF1
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WB
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IF-Cell
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IF-Tissue
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IP
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IHC-P
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FC
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Human
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Mouse
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
hUPF1 Recombinant Rabbit Monoclonal Antibody [JG38-44] (ET7108-70)
Immunogen
Recombinant protein within human hupf1 aa 1-120 / 1129.
Host
Rabbit
Positive Control
PC-3M cell lysates, A431, LOVO, PC-3M, human liver tissue, mouse liver tissue, rat liver tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG38-44
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
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IP
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1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 124 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
hUPF1
SYNONYMS
ATP dependent helicase RENT1 antibody;ATP-dependent helicase RENT1 antibody;Delta helicase antibody;FLJ43809 antibody;FLJ46894 antibody;HUPF 1 antibody;hUpf1 antibody;KIAA0221 antibody;Nonsense mRNA reducing factor 1 antibody;NORF 1 antibody;NORF1 antibody;pNORF 1 antibody;pNORF1 antibody;Regulator of nonsense transcripts 1 antibody;RENT 1 antibody;RENT1 antibody;RENT1_HUMAN antibody;Smg 2 antibody;Smg 2 homolog nonsense mediated mRNA decay factor antibody;UP Frameshift 1 antibody;Up frameshift mutation 1 homolog (S. cerevisiae) antibody;Up frameshift mutation 1 homolog antibody;Up frameshift suppressor 1 homolog antibody;Up-frameshift suppressor 1 homolog antibody;UPF 1 antibody;UPF 1 regulator of nonsense transcripts homolog antibody;upf1 antibody;UPF1 regulator of nonsense transcripts homolog antibody;UPF1 RNA helicase and ATPase antibody;Yeast Upf1p homolog antibody
SEQUENCE SIMILARITIES
Belongs to the DNA2/NAM7 helicase family.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by SMG1; required for formation of mRNA surveillance complexes.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent 1 and Rent 2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.
CITATIONS
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A transcription‐independent mechanism determines rapid periodic fluctuations of BRCA1 expression
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WB
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Human
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Citation
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