PRODUCT CODE: ET7108-70

hUPF1 Recombinant Rabbit Monoclonal Antibody [JG38-44] (ET7108-70)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1,000 dilution.
  • Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1,000 dilution.
  • ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

hUPF1 Recombinant Rabbit Monoclonal Antibody [JG38-44] (ET7108-70)

Immunogen

Recombinant protein within human hupf1 aa 1-200.

Host

Rabbit

Positive Control

PC-3M, A431, LOVO, human colon tissue, human prostate cancer tissue, human kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG38-44

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

124 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

hUPF1

SYNONYMS

ATP dependent helicase RENT1 antibody; ATP-dependent helicase RENT1 antibody; Delta helicase antibody; FLJ43809 antibody; FLJ46894 antibody; HUPF 1 antibody; hUpf1 antibody; KIAA0221 antibody; Nonsense mRNA reducing factor 1 antibody; NORF 1 antibody; NORF1 antibody; pNORF 1 antibody; pNORF1 antibody; Regulator of nonsense transcripts 1 antibody; RENT 1 antibody; RENT1 antibody; RENT1_HUMAN antibody; Smg 2 antibody; Smg 2 homolog nonsense mediated mRNA decay factor antibody; UP Frameshift 1 antibody; Up frameshift mutation 1 homolog (S. cerevisiae) antibody; Up frameshift mutation 1 homolog antibody; Up frameshift suppressor 1 homolog antibody; Up-frameshift suppressor 1 homolog antibody; UPF 1 antibody; UPF 1 regulator of nonsense transcripts homolog antibody; upf1 antibody; UPF1 regulator of nonsense transcripts homolog antibody; UPF1 RNA helicase and ATPase antibody; Yeast Upf1p homolog antibody

SEQUENCE SIMILARITIES

Belongs to the DNA2/NAM7 helicase family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by SMG1; required for formation of mRNA surveillance complexes.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent 1 and Rent 2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.