PRODUCT CODE: ET7107-60

Huntingtin Recombinant Rabbit Monoclonal Antibody [JB89-34] (ET7107-60)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Huntingtin on A431 cell lysates using anti-Huntingtin antibody at 1/500 dilution.
  • Western blot analysis of Huntingtin on A431 cell lysates using anti-Huntingtin antibody at 1/500 dilution.
  • Western blot analysis of Huntingtin on SH-SY5Y cell lysates using anti-Huntingtin antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Huntingtin on A431 cell lysates using anti-Huntingtin antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Huntingtin Recombinant Rabbit Monoclonal Antibody [JB89-34] (ET7107-60)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue, human colon cancer tissue, human breast tissue, mouse epididymis tissue, SH-SY-5Y, A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB89-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

347 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • ICC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

Huntingtin

SYNONYMS

AI256365 antibody; C430023I11Rik antibody; HD antibody; HD protein antibody; HD_HUMAN antibody; HDH antibody; HTT antibody; Huntingtin antibody; HUNTINGTON CHOREA antibody; Huntington disease protein antibody; Huntington's disease protein homolog antibody; IT 15 antibody; IT15 antibody; OTTMUSP00000026909 antibody; ZHD antibody

SEQUENCE SIMILARITIES

Belongs to the huntingtin family.

TISSUE SPECIFICITY

Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

POST-TRANSLATIONAL MODIFICATION

[Huntingtin]: Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis.; [Huntingtin]: Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.; [Huntingtin]: Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity.; [Huntingtin, myristoylated N-terminal fragment]: Myristoylated at Gly-551, following proteolytic cleavage at Asp-550.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The huntingtin gene, also called the HTT or HD (Huntington disease) gene, is the IT15 ("interesting transcript 15") gene, which codes for a protein called the huntingtin protein. It is variable in its structure, as the many polymorphisms of the gene can lead to variable numbers of glutamine residues present in the protein. The mass of huntingtin protein is dependent largely on the number of glutamine residues it has, the predicted mass is around 350 kDa. Normal huntingtin is generally accepted to be 3144 amino acids in size. The exact function of this protein is not known, but it plays an important role in nerve cells. Within cells, huntingtin may be involved in signaling, transporting materials, binding proteins and other structures, and protecting against programmed cell death (apoptosis). The huntingtin protein is required for normal development before birth. It is expressed in many tissues in the body, with the highest levels of expression seen in the brain.