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Human VEGF enzyme-linked immunoassay kit (EH0075)

Price: Regular price $499.00

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Catalog# EH0075

Assay type Sandwich ELISA

Standard curve range 31.25~2000 pg/ml

Sensitivity 1.04 pg/ml (50 μl)；3.21 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02750.02640.0270
31.250.11900.10820.11360.0867
62.500.19910.20400.20160.1746
125.000.31590.28630.30110.2742
250.000.55210.60450.57830.5514
500.001.15601.22601.19101.1641
1000.002.31702.21302.26502.2381
2000.003.62803.49903.56353.5366

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)53.2229.1694.531.6160.6438.1
Standard Deviation3.613.338.42.18.213.6
Coefficient of Variation(%)6.85.85.56.55.13.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3025.21-450.32100185.52

Sensitivity

1.04 pg/ml (50 μl)；3.21 pg/ml (10 μl)

Standard curve range

31.25~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human VEGF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 94% to 119% with an overall mean recovery of 105%.

Specifications

Product Type

ELISA Kit

Product Name

Human VEGF enzyme-linked immunoassay kit (EH0075)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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