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Human TGF-α enzyme-linked immunoassay kit (EH0059)

Price: Regular price $499.00

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Catalog# EH0059

Assay type Sandwich ELISA

Standard curve range 4.12~3000 pg/ml

Sensitivity 1.91 pg/ml (50 μl)；3.89 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.04220.03820.0402
4.120.04710.05270.04990.0439
12.350.07260.07120.07190.0659
37.040.12850.12850.12850.1225
111.110.30910.31350.31130.3053
333.330.75660.77920.76790.7619
1000.001.75601.66901.71251.7065
3000.003.13503.15303.14403.1380

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)59.6296.21122.058.2320.61180.8
Standard Deviation2.720.874.42.316.340.8
Coefficient of Variation(%)4.57.06.64.05.13.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-25.28509.57

Sensitivity

1.91 pg/ml (50 μl)；3.89 pg/ml (10 μl)

Standard curve range

4.12~3000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human TGF-α into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 104% to 126% with an overall mean recovery of 114%.

Specifications

Product Type

ELISA Kit

Product Name

Human TGF-α enzyme-linked immunoassay kit (EH0059)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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