Human Serum Albumin Mouse Monoclonal Antibody [8-A3] (M0809-4)

Western blot analysis on HSA protein using anti-HSA Mouse mAb (Cat. # M0809-4).

Applications

REACTIVITY

Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Human Serum Albumin Mouse Monoclonal Antibody [8-A3] (M0809-4)

Immunogen

Protein

Host

Mouse

Positive Control

Purified HSA protein

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

8-A3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70kDa

Isotype

IgG1

APPLICATION DILUTION

WB
1:2,000

TARGET

UNIPROT #

P02768

PROTEIN NAME

Human Serum Albumin

SYNONYMS

alb antibody; ALBU_HUMAN antibody; Albumin (32 AA) antibody; Albumin (AA 34) antibody; Albumin antibody; Analbuminemia antibody; Bisalbuminemia antibody; Cell growth inhibiting protein 42 antibody; DKFZp779N1935 antibody; Dysalbuminemic hyperthyroxinemia antibody; Growth inhibiting protein 20 antibody; HSA antibody; Hyperthyroxinemia dysalbuminemic antibody; PRO0883 antibody; PRO0903 antibody; PRO1341 antibody; Serum albumin antibody

SEQUENCE SIMILARITIES

Belongs to the ALB/AFP/VDB family.

TISSUE SPECIFICITY

Plasma.

POST-TRANSLATIONAL MODIFICATION

Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.; Glycated in diabetic patients.; Phosphorylated by FAM20C in the extracellular medium.; Acetylated on Lys-223 by acetylsalicylic acid.

SUBCELLULAR LOCATION

Secreted

FUNCTION

HSA is the most common protein in serum. It is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. HSA binds a wide variety of hydrophobic ligands including fatty acids, bilirubin, thyroxine and hemin.

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4] (M0910-2)

Applications

WB
ELISA
IP

REACTIVITY

Goat

Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Thymidine phosphorylase Mouse Monoclonal Antibody [A1A8] (HA600036)

Applications

REACTIVITY

Human

Western blot analysis of Thymidine phosphorylase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600036, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MMAF Mouse Monoclonal Antibody [A5G11] (HA600035)

Applications

ELISA

REACTIVITY

The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Applications

ELISA

REACTIVITY

The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

IGF1R Mouse Monoclonal Antibody [D5-G2] (HA600033)

Applications

REACTIVITY

Human

Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat