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Human PDGF-AA enzyme-linked immunoassay kit (EH0058)

Price: Regular price $499.00

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Catalog# EH0058

Assay type Sandwich ELISA

Standard curve range 0.31~20 ng/ml

Sensitivity 0.10 ng/ml (50 μl)；0.17 ng/ml (10 μl)

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.23110.24030.2357
0.310.29420.30110.29770.0619
0.630.32710.37830.35270.1170
1.250.43280.44530.43910.2034
2.500.61210.61720.61470.3790
5.001.03001.08301.05650.8208
10.001.80601.87301.83951.6038
20.003.05403.07903.06652.8308

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)0.412.166.480.502.356.85
Standard Deviation0.030.110.290.030.150.35
Coefficient of Variation(%)6.75.24.57.06.25.1

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum3021.10-57.8910033.58

Sensitivity

0.10 ng/ml (50 μl)；0.17 ng/ml (10 μl)

Standard curve range

0.31~20 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human PDGF-AA into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 98% to 112% with an overall mean recovery of 106%.

Specifications

Product Type

ELISA Kit

Product Name

Human PDGF-AA enzyme-linked immunoassay kit (EH0058)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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